Review



mouse neuronal cell line pc12  (Procell Inc)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Procell Inc mouse neuronal cell line pc12
    Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in <t>PC12</t> cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.
    Mouse Neuronal Cell Line Pc12, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse neuronal cell line pc12/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    mouse neuronal cell line pc12 - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Activation of STING signaling aggravates chronic alcohol exposure‐induced cognitive impairment by increasing neuroinflammation and mitochondrial apoptosis"

    Article Title: Activation of STING signaling aggravates chronic alcohol exposure‐induced cognitive impairment by increasing neuroinflammation and mitochondrial apoptosis

    Journal: CNS Neuroscience & Therapeutics

    doi: 10.1111/cns.14689

    Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in PC12 cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.
    Figure Legend Snippet: Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in PC12 cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.

    Techniques Used: Activation Assay, Western Blot, Expressing, Control, Flow Cytometry, Membrane, Gene Expression



    Similar Products

    mouse neuronal cell line pc12  (ATCC)
    98
    ATCC mouse neuronal cell line pc12
    Mouse Neuronal Cell Line Pc12, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse neuronal cell line pc12/product/ATCC
    Average 98 stars, based on 1 article reviews
    mouse neuronal cell line pc12 - by Bioz Stars, 2026-03
    98/100 stars
    		  Buy from Supplier
    mouse neuronal cell line pc12  (Procell Inc)
    90
    Procell Inc mouse neuronal cell line pc12
    Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in <t>PC12</t> cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.
    Mouse Neuronal Cell Line Pc12, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse neuronal cell line pc12/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    mouse neuronal cell line pc12 - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in PC12 cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.

    Journal: CNS Neuroscience & Therapeutics

    Article Title: Activation of STING signaling aggravates chronic alcohol exposure‐induced cognitive impairment by increasing neuroinflammation and mitochondrial apoptosis

    doi: 10.1111/cns.14689

    Figure Lengend Snippet: Activation of STING signaling induced by alcohol in neurons led to mitochondrial apoptosis. (A, B) Representative immunoblot images and quantitative analyses of the expression of STING pathway‐associated genes in PC12 cells treated with control and EtOH (300 mM) ( n = 3). (C) Representative immunoblot images showing the expression of STING signaling pathway‐associated genes in primary neurons treated with various concentrations of alcohol (0, 100, 200, 300 mM). (D, E) Representative images and quantitative analyses of apoptotic PC12 cells in the different groups, as measured by flow cytometry ( n = 4, DMXAA (50 μg/mL)). (F) Quantitative analyses of the activities of caspase 3, caspase 9, and caspase 8 in PC12 cells in the different groups ( n = 3, DMXAA (50 μg/mL)). (G, I) Effects of DMXAA on the mitochondrial membrane potential of PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). (H, J) Effects of DMXAA on ROS production in PC12 cells treated with alcohol, as measured by flow cytometry using JC‐1 ( n = 3, DMXAA (50 μg/mL)). Scale bar = 100 μm. (K, L) Representative images and quantitative analyses of TBK1, p‐TBK1 and mitochondrial apoptosis‐associated gene expression in the different groups ( n = 3, DMXAA (50 μg/mL)). The data are expressed as the mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001; ns, nonsignificant.

    Article Snippet: The mouse microglial cell line BV2 and the mouse neuronal cell line PC12, purchased from Procell Life, China, were cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (FBS, Excellbio, China) and 1% penicillin and streptomycin (Gibco, USA) at 37°C and 5% CO2.

    Techniques: Activation Assay, Western Blot, Expressing, Control, Flow Cytometry, Membrane, Gene Expression